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A bimolecular affinity purification method under denaturing conditions for rapid isolation of a ubiquitinated protein for mass spectrometry analysis

JOURNAL ARTICLE published August 2010 in Nature Protocols

Authors: Gabriel N Maine | Haiying Li | Iram W Zaidi | Venkatesha Basrur | Kojo S J Elenitoba-Johnson | Ezra Burstein

Total low-molecular-weight antioxidants as a summary parameter, quantified in biological samples by a chemiluminescence inhibition assay

JOURNAL ARTICLE published October 2010 in Nature Protocols

Authors: Livia Saleh | Christoph Plieth

Standardized production of hPSC-derived cardiomyocyte aggregates in stirred spinner flasks

JOURNAL ARTICLE published 28 March 2024 in Nature Protocols

Research funded by Deutsche Forschungsgemeinschaft (REBIRTH EXC 62/2, ZW64/4-2) | Bundesministerium für Bildung und Forschung (01EK1601A, 13XP5092B, 031L0249, 01EK2108A) | Niedersächsische Ministerium für Wissenschaft und Kultur (ZN3340, ZN4092) | European Commission (101056712)

Authors: Nils Kriedemann | Wiebke Triebert | Jana Teske | Mira Mertens | Annika Franke | Kevin Ullmann | Felix Manstein | Lika Drakhlis | Alexandra Haase | Caroline Halloin | Ulrich Martin | Robert Zweigerdt

Magnetically guided virus stamping for the targeted infection of single cells or groups of cells

JOURNAL ARTICLE published November 2019 in Nature Protocols

Authors: Rajib Schubert | Sophie Herzog | Stuart Trenholm | Botond Roska | Daniel J. Müller

Protein higher-order-structure determination by fast photochemical oxidation of proteins and mass spectrometry analysis

JOURNAL ARTICLE published December 2020 in Nature Protocols

Research funded by U.S. Department of Health & Human Services | NIH | Office of Extramural Research, National Institutes of Health (P41GM103422,R24GM136766,1S10OD016298)

Authors: Xiaoran Roger Liu | Don L. Rempel | Michael L. Gross

In situ observation of conformational dynamics and protein ligand–substrate interactions in outer-membrane proteins with DEER/PELDOR spectroscopy

JOURNAL ARTICLE published August 2019 in Nature Protocols

Authors: Benesh Joseph | Eva A. Jaumann | Arthur Sikora | Katja Barth | Thomas F. Prisner | David S. Cafiso

Profiling of DNA replication timing in unsynchronized cell populations

JOURNAL ARTICLE published November 2006 in Nature Protocols

Authors: Véronique Azuara

Creating self-illuminating quantum dot conjugates

JOURNAL ARTICLE published August 2006 in Nature Protocols

Authors: Min-Kyung So | Andreas M Loening | Sanjiv S Gambhir | Jianghong Rao

Subretinal injection in mice to study retinal physiology and disease

JOURNAL ARTICLE published June 2022 in Nature Protocols

Research funded by U.S. Department of Health & Human Services | National Institutes of Health (R01EY028027)

Authors: Peirong Huang | Siddharth Narendran | Felipe Pereira | Shinichi Fukuda | Yosuke Nagasaka | Ivana Apicella | Praveen Yerramothu | Kenneth M. Marion | Xiaoyu Cai | Srinivas R. Sadda | Bradley D. Gelfand | Jayakrishna Ambati

Preparation of supramolecular hydrogel–enzyme hybrids exhibiting biomolecule-responsive gel degradation

JOURNAL ARTICLE published September 2016 in Nature Protocols

Authors: Hajime Shigemitsu | Takahiro Fujisaku | Shoji Onogi | Tatsuyuki Yoshii | Masato Ikeda | Itaru Hamachi

An expedient, facile and simple one-pot synthesis of 2-methylenealkanoates and alkanenitriles from the Baylis–Hillman bromides in aqueous media

JOURNAL ARTICLE published November 2007 in Nature Protocols

Authors: Deevi Basavaiah | Kanumuri Ramesh Reddy | Nagaswamy Kumaragurubaran

Reverse MAPPIT detects disruptors of protein-protein interactions in human cells

JOURNAL ARTICLE published June 2006 in Nature Protocols

Authors: Irma Lemmens | Sam Lievens | Sven Eyckerman | Jan Tavernier

Quantitation of human mammary epithelial stem cells with in vivo regenerative properties using a subrenal capsule xenotransplantation assay

JOURNAL ARTICLE published December 2010 in Nature Protocols

Authors: Peter Eirew | John Stingl | Connie J Eaves

A preclinical xenograft model of prostate cancer using human tumors

JOURNAL ARTICLE published May 2013 in Nature Protocols

Authors: Mitchell G Lawrence | Renea A Taylor | Roxanne Toivanen | John Pedersen | Sam Norden | David W Pook | Mark Frydenberg | Melissa M Papargiris | Birunthi Niranjan | Michelle G Richards | Hong Wang | Anne T Collins | Norman J Maitland | Gail P Risbridger | Australian Prostate Cancer BioResource

Synthesis of a highly Zn2+-selective cyanine-based probe and its use for tracing endogenous zinc ions in cells and organisms

JOURNAL ARTICLE published June 2014 in Nature Protocols

Authors: Zhiqian Guo | Gun-Hee Kim | Juyoung Yoon | Injae Shin

Cryo-EM of macromolecular assemblies at near-atomic resolution

JOURNAL ARTICLE published October 2010 in Nature Protocols

Authors: Matthew L Baker | Junjie Zhang | Steven J Ludtke | Wah Chiu

Genome-wide mapping of endogenous G-quadruplex DNA structures by chromatin immunoprecipitation and high-throughput sequencing

JOURNAL ARTICLE published March 2018 in Nature Protocols

Authors: Robert Hänsel-Hertsch | Jochen Spiegel | Giovanni Marsico | David Tannahill | Shankar Balasubramanian

Preparation of glycoconjugates from unprotected carbohydrates for protein-binding studies

JOURNAL ARTICLE published November 2017 in Nature Protocols

Authors: Christian T Hjuler | Nicolai N Maolanon | Jørgen Sauer | Jens Stougaard | Mikkel B Thygesen | Knud J Jensen

Calculating absolute and relative protein abundance from mass spectrometry-based protein expression data

JOURNAL ARTICLE published August 2008 in Nature Protocols

Authors: Christine Vogel | Edward M Marcotte

Selective monitoring of ubiquitin signals with genetically encoded ubiquitin chain–specific sensors

JOURNAL ARTICLE published July 2013 in Nature Protocols

Authors: Sjoerd J L van Wijk | Evgenij Fiškin | Ivan Dikic