Preparation of fatty acid solutions exerts significant impact on experimental outcomes in cell culture models of lipotoxicity

JOURNAL ARTICLE published 10 January 2022 in Biology Methods and Protocols

Authors: Axel Römer | Divya Rawat | Thomas Linn | Sebastian F Petry

BIOMAP: A Home for All Biology Methods

JOURNAL ARTICLE published 1 March 2016 in Biology Methods and Protocols

Authors: Silvia Ayora

Single well, single-common primer pair, dual probe, duplex qPCR assay for the quantification of mRNA splicing variants

JOURNAL ARTICLE published 22 January 2021 in Biology Methods and Protocols

Authors: Janice Wang | Winifred P Wong | Emma O Link | Shantel Olivares | Cade T Adelman | Anne S Henkel | Malek El Muayed

Covalent modification of primers improves PCR amplification specificity and yield

JOURNAL ARTICLE published 1 January 2017 in Biology Methods and Protocols

Authors: Nancy J Schoenbrunner | Amar P Gupta | Karen K Y Young | Stephen G Will

Serum metallomics reveals insights into the associations of elements with the progression of preleukemic diseases toward acute leukemia

JOURNAL ARTICLE published 11 January 2024 in Biology Methods and Protocols

Authors: Amna Jabbar Siddiqui | Noman Khan | Kauser Fatima | Sabiha Farooq | Muhammad Ramzan | Hesham R El-Seedi | Jalal Uddin | Abdullatif Bin Muhsinah | Syed Ghulam Musharraf

Optimizing simple calreticulin upregulation strategies in Caenorhabditis elegans

JOURNAL ARTICLE published 10 January 2023 in Biology Methods and Protocols

Authors: Ana Guijarro-Hernández | Cristina Hurtado | José Luis Vizmanos

Rapid and inexpensive whole-genome sequencing of SARS-CoV-2 using 1200 bp tiled amplicons and Oxford Nanopore Rapid Barcoding

JOURNAL ARTICLE published 1 January 2020 in Biology Methods and Protocols

Authors: Nikki E Freed | Markéta Vlková | Muhammad B Faisal | Olin K Silander

Construction of an overexpression library for Mycobacterium tuberculosis

JOURNAL ARTICLE published 1 January 2018 in Biology Methods and Protocols

Research funded by Bill and Melinda Gates Foundation (42844,OPP1024038) | National Institutes of Health (R01AI099188)

Authors: Eduard Melief | Rachel Kokoczka | Megan Files | Mai Ann Bailey | Torey Alling | Hongye Li | James Ahn | Ayesha Misquith | Aaron Korkegian | David Roberts | James Sacchettini | Tanya Parish

MLBioIGE: integration and interplay of machine learning and bioinformatics approach to identify the genetic effect of SARS-COV-2 on idiopathic pulmonary fibrosis patients

JOURNAL ARTICLE published 10 January 2022 in Biology Methods and Protocols

Authors: Sk Tanzir Mehedi | Kawsar Ahmed | Francis M Bui | Musfikur Rahaman | Imran Hossain | Tareq Mahmud Tonmoy | Rakibul Alam Limon | Sobhy M Ibrahim | Mohammad Ali Moni

Preparation and processing of dried blood spots for microRNA sequencing

JOURNAL ARTICLE published 10 January 2023 in Biology Methods and Protocols

Research funded by National Institute on Drug Abuse (R01DA037911) | National Institute of Mental Health (R37MH101495,K01MH117442)

Authors: Alice Morgunova | Pascal Ibrahim | Gary Gang Chen | Saché M Coury | Gustavo Turecki | Michael J Meaney | Anthony Gifuni | Ian H Gotlib | Corina Nagy | Tiffany C Ho | Cecilia Flores

Teaching computational genomics and bioinformatics on a high performance computing cluster—a primer

JOURNAL ARTICLE published 10 January 2022 in Biology Methods and Protocols

Research funded by National Institutes of Health (1R15GM143700-01)

Authors: Arun Sethuraman

Establishing an effective gene knockdown system using cultured cells of the model fish medaka (Oryzias latipes)

JOURNAL ARTICLE published 10 January 2022 in Biology Methods and Protocols

Authors: Kosuke Zenke | Yasushi Okinaka

Wildlife pathogen detection: evaluation of alternative DNA extraction protocols

JOURNAL ARTICLE published 22 January 2021 in Biology Methods and Protocols

Research funded by Deutsche Forschungsgemeinschaft (DFG) to J.S.-P. (SA 3786/1-1)

Authors: Vasiliki Mantzana-Oikonomaki | Martine Maan | Joana Sabino-Pinto

The COVID-19 Community Research Partnership: a multistate surveillance platform for characterizing the epidemiology of the SARS-CoV-2 pandemic

JOURNAL ARTICLE published 10 January 2022 in Biology Methods and Protocols

Research funded by Centers for Disease Control and Prevention (#75D30120C08405) | Department of Health and Human Services (NC DHHS GTS #49927)

Authors: The COVID-19 Community Research Partnership | Thomas F Wierzba | John Walton Sanders | David Herrington | Mark A Espeland | John Williamson | Morgana Mongraw-Chaffin | Alain Bertoni | Martha A Alexander-Miller | Paola Castri | Allison Mathews | Iqra Munawar | Austin Lyles Seals | Brian Ostasiewski | Christine Ann Pittman Ballard | Metin Gurcan | Alexander Ivanov | Giselle Melendez Zapata | Marlena Westcott | Karen Blinson | Laura Blinson | Mark Mistysyn | Donna Davis | Lynda Doomy | Perrin Henderson | Alicia Jessup | Kimberly Lane | Beverly Levine | Jessica McCanless | Sharon McDaniel | Kathryn Melius | Christine O'Neill | Angelina Pack | Ritu Rathee | Scott Rushing | Jennifer Sheets | Sandra Soots | Michele Wall | Samantha Wheeler | John White | Lisa Wilkerson | Rebekah Wilson | Kenneth Wilson | Deb Burcombe | Georgia Saylor | Megan Lunn | Karina Ordonez | Ashley O'Steen | Leigh Wagner | Michael S Runyon | Lewis H McCurdy | Michael A Gibbs | Yhenneko J Taylor | Lydia Calamari | Hazel Tapp | Amina Ahmed | Michael Brennan | Lindsay Munn | Keerti L Dantuluri | Timothy Hetherington | Lauren C Lu | Connell Dunn | Melanie Hogg | Andrea Price | Marina Leonidas | Melinda Manning | Whitney Rossman | Frank X Gohs | Anna Harris | Jennifer S Priem | Pilar Tochiki | Nicole Wellinsky | Crystal Silva | Tom Ludden | Jackeline Hernandez | Kennisha Spencer | Laura McAlister | William Weintraub | Kristen Miller | Chris Washington | Allison Moses | Sarahfaye Dolman | Julissa Zelaya-Portillo | John Erkus | Joseph Blumenthal | Ronald E Romero Barrientos | Sonita Bennett | Shrenik Shah | Shrey Mathur | Christian Boxley | Paul Kolm | Ella Franklin | Naheed Ahmed | Moira Larsen | Richard Oberhelman | Joseph Keating | Patricia Kissinger | John Schieffelin | Joshua Yukich | Andrew Beron | Johanna Teigen | Karen Kotloff | Wilbur H Chen | DeAnna Friedman-Klabanoff | Andrea A Berry | Helen Powell | Lynnee Roane | Reva Datar | Colleen Reilly | Adolfo Correa | Bhagyashri Navalkele | Yuan-I Min | Alexandra Castillo | Lori Ward | Robert P Santos | Pramod Anugu | Yan Gao | Jason Green | Ramona Sandlin | Donald Moore | Lemichal Drake | Dorothy Horton | Kendra L Johnson | Michael Stover | William H Lagarde | LaMonica Daniel | Patrick D Maguire | Charin L Hanlon | Lynette McFayden | Isaura Rigo | Kelli Hines | Lindsay Smith | Monique Harris | Belinda Lissor | Vivian Cook | Maddy Eversole | Terry Herrin | Dennis Murphy | Lauren Kinney | Polly Diehl | Nicholas Abromitis | Tina St Pierre | Bill Heckman | Denise Evans | Julian March | Ben Whitlock | Wendy Moore | Sarah Arthur | Joseph Conway | Thomas R Gallaher | Mathew Johanson | Sawyer Brown | Tina Dixon | Martha Reavis | Shakira Henderson | Michael Zimmer | Danielle Oliver | Kasheta Jackson | Monica Menon | Brandon Bishop | Rachel Roeth | Robin King-Thiele | Terri S Hamrick | Abdalla Ihmeidan | Amy Hinkelman | Chika Okafor | Regina B Bray Brown | Amber Brewster | Danius Bouyi | Katrina Lamont | Kazumi Yoshinaga | Poornima Vinod | A Suman Peela | Giera Denbel | Jason Lo | Mariam Mayet-Khan | Akash Mittal | Reena Motwani | Mohamed Raafat | Evan Schultz | Aderson Joseph | Aalok Parkeh | Dhara Patel | Babar Afridi | Diane Uschner | Sharon L Edelstein | Michele Santacatterina | Greg Strylewicz | Brian Burke | Mihili Gunaratne | Meghan Turney | Shirley Qin Zhou | Ashley H Tjaden | Lida Fette | Asare Buahin | Matthew Bott | Sophia Graziani | Ashvi Soni | Guoqing Diao | Jone Renteria | Christopher Mores | Abigail Porzucek | Rebecca Laborde | Pranav Acharya | Lucy Guill | Danielle Lamphier | Anna Schaefer | William M Satterwhite | Anne McKeague | Johnathan Ward | Diana P Naranjo | Nana Darko | Kimberly Castellon | Ryan Brink | Haris Shehzad | Derek Kuprianov | Douglas McGlasson | Devin Hayes | Sierra Edwards | Stephane Daphnis | Britnee Todd | Atira Goodwin | Ruth Berkelman | Kimberly Hanson | Scott Zeger | Johns Hopkins | Cavan Reilly | Kathy Edwards | Helene Gayle | Stephen Redd

Teaching at the intersection of science and society: An activity on healthcare disparities

JOURNAL ARTICLE published 11 January 2024 in Biology Methods and Protocols

Research funded by National Science Foundation (DGE-2327488)

Authors: Paula E Adams | Enya Granados | Abby E Beatty | Cissy J Ballen

Optimization and biological validation of an in vitro assay using the transfected Dm28c/pLacZ Trypanosoma cruzi strain

JOURNAL ARTICLE published 22 January 2021 in Biology Methods and Protocols

Authors: Julián Ernesto Nicolás Gulin | Daniela Marisa Rocco | Victoria Alonso | Pamela Cribb | Jaime Altcheh | Facundo García-Bournissen

Prediction of risk-associated genes and high-risk liver cancer patients from their mutation profile: benchmarking of mutation calling techniques

JOURNAL ARTICLE published 10 January 2022 in Biology Methods and Protocols

Authors: Sumeet Patiyal | Anjali Dhall | Gajendra P S Raghava

Plasma or serum? A qualitative study on rodents and humans using high-throughput microRNA sequencing for circulating biomarkers

JOURNAL ARTICLE published 1 January 2019 in Biology Methods and Protocols

Authors: Thibault Dufourd | Noémie Robil | David Mallet | Carole Carcenac | Sabrina Boulet | Sonia Brishoual | Emilie Rabois | Jean-Luc Houeto | Pierre de la Grange | Sebastien Carnicella

An optimized HEK293T cell expansion protocol using a hollow-fiber bioreactor system

JOURNAL ARTICLE published 10 January 2023 in Biology Methods and Protocols

Authors: Nathan D Frank | Mindy Miller | Dalip Sethi

Development of a fixation-free fluorescence in situ hybridization for the detection of Salmonella species

JOURNAL ARTICLE published 1 January 2020 in Biology Methods and Protocols

Research funded by Commonwealth Scholarship Commission, UK (NGCA -2011-74)

Authors: Oluwawemimo Adebowale | Liam Good