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A comparison of the efficiency of RNA extraction from extracellular vesicles using the Qiagen RNeasy MinElute versus Enzymax LLC RNA Tini Spin columns and qPCR of miRNA

JOURNAL ARTICLE published 22 January 2021 in Biology Methods and Protocols

Authors: Rachael Anne Dunlop | Sandra Anne Banack | Paul Alan Cox

Quantifying the invasion and migration ability of cancer cells with a 3D Matrigel drop invasion assay

JOURNAL ARTICLE published 22 January 2021 in Biology Methods and Protocols

Research funded by Canary Foundation, the National Institutes of Health/National Cancer Institute (R37CA240822,R01CA244281) | NIH (S10 OD023518-01A1) | Celigo S Imaging Cytometer (200-BFFL-S)

Authors: Merve Aslan | En-Chi Hsu | Shiqin Liu | Tanya Stoyanova

Desktop scanning electron microscopy in plant–insect interactions research: a fast and effective way to capture electron micrographs with minimal sample preparation

JOURNAL ARTICLE published 10 January 2022 in Biology Methods and Protocols

Authors: Sakshi Watts | Ishveen Kaur | Sukhman Singh | Bianca Jimenez | Jesus Chavana | Rupesh Kariyat

OptM: estimating the optimal number of migration edges on population trees using Treemix

JOURNAL ARTICLE published 22 January 2021 in Biology Methods and Protocols

Authors: Robert R Fitak

A fast, sensitive and cost-effective method for nucleic acid detection using non-radioactive probes

JOURNAL ARTICLE published 1 January 2018 in Biology Methods and Protocols

Authors: David Viterbo | Astrid Marchal | Valentine Mosbach | Lucie Poggi | Wilhelm Vaysse-Zinkhöfer | Guy-Franck Richard

Identification of stable reference genes for lipopolysaccharide-stimulated macrophage gene expression studies

JOURNAL ARTICLE published 1 March 2016 in Biology Methods and Protocols

Authors: Roshini Kalagara | Weimin Gao | Honor L. Glenn | Colleen Ziegler | Laura Belmont | Deirdre R. Meldrum

Concomitant prediction of environmental fate and toxicity of chemical compounds

JOURNAL ARTICLE published 1 January 2020 in Biology Methods and Protocols

Research funded by the Spanish Ministry of Economy and Competitiveness with European Regional Development Fund (SAF2016-78041-C2-2-R) | the SETH (RTI2018-095584-B-C42, MINECO/FEDER) | SyCoLiM (ERA-COBIOTECH 2018—PCI2019-111859-2) | Projects of the Spanish Ministry of Science and Innovation, the MADONNA (H2020-FET-OPEN-RIA-2017-1-766975) | BioRoboost (H2020-NMBP-BIO-CSA-2018-820699) | SynBio4Flav (H2020-NMBP-TR-IND/H2020-NMBP-BIO-2018-814650) | MIX-UP (MIX-UP H2020-BIO-CN-2019-870294) | Contracts of the European Union, as well as the InGEMICS-CM (S2017/BMD-3691,Project of the Comunidad de Madrid—European Structural and Investment Funds (FSE, FECER))

Authors: Juan Antonio Garcia-Martin | Max Chavarría | Victor de Lorenzo | Florencio Pazos

Effectiveness of a community-based intervention (Konga model) to address the factors contributing to viral load suppression among children living with HIV in Tanzania: a cluster-randomized clinical trial protocol

JOURNAL ARTICLE published 10 January 2022 in Biology Methods and Protocols

Authors: Mageda Kihulya | Leornard K Katalambula | Ntuli A Kapologwe | Pammla Petrucka

COVID’s long shadow: How SARS-CoV-2 infection, COVID-19 severity, and vaccination status affect long-term cognitive performance and health

JOURNAL ARTICLE published 10 January 2023 in Biology Methods and Protocols

Authors: Jaroslav Flegr | Ashkan Latifi

Surgical method to prevent early death of neonatal rat pups with Hirschsprung disease, thus permitting development of long-term therapeutic approaches

JOURNAL ARTICLE published 10 January 2022 in Biology Methods and Protocols

Research funded by Takeda Pharmaceuticals and the Medical Research Future Fund (APP2009049)

Authors: Lincon A Stamp | Enie Lei | Jamie J M Liew | Ruslan V Pustovit | Marlene M Hao | David H Croaker | John B Furness | Cameron D Adams

A two-step PCR assembly for construction of gene variants across large mutational distances

JOURNAL ARTICLE published 22 January 2021 in Biology Methods and Protocols

Research funded by IIT Kharagpur and an Early career research (ECR/2017/002328)

Authors: Shreya Routh | Anamika Acharyya | Riddhiman Dhar

MultiPep: a hierarchical deep learning approach for multi-label classification of peptide bioactivities

JOURNAL ARTICLE published 23 November 2021 in Biology Methods and Protocols

A new method for measuring the size of nematodes using image processing

JOURNAL ARTICLE published 1 January 2019 in Biology Methods and Protocols

Authors: David G H Andrews

Sera of patients infected by earlier lineages of SARS-CoV-2 are capable to neutralize later emerged variants of concern

JOURNAL ARTICLE published 10 January 2022 in Biology Methods and Protocols

Research funded by Coordination of Health Surveillance and Reference Laboratories of Oswaldo Cruz Foundation (402457/2020-9,403276/2020-9)

Authors: Alex Pauvolid-Corrêa | Braulia Costa Caetano | Ana Beatriz Machado | Mia Araújo Ferreira | Natalia Valente | Thayssa Keren Neves | Kim Geraldo | Fernando Motta | Valdiléa Gonçalves Veloso dos Santos | Beatriz Grinsztejn | Marilda Mendonça Siqueira | Paola Cristina Resende

Detection of SARS-CoV-2 B.1.1.529 (Omicron) variant by SYBR Green-based RT-qPCR

JOURNAL ARTICLE published 11 January 2024 in Biology Methods and Protocols

Authors: Fadi Abdel-Sater | Rawan Makki | Alia Khalil | Nader Hussein | Nada Borghol | Ziad Abi Khattar | Aline Hamade | Nathalie Khreich | Mahoumd El Homsi | Hussein Kanaan | Line Raad | Najwa Skafi | Fatima Al-Nemer | Zeinab Ghandour | Nabil El-Zein | Mhamad Abou-Hamdan | Haidar Akl | Eva Hamade | Bassam Badran | Kassem Hamze

A highly sensitive trap vector system for isolating reporter cells and identification of responsive genes

JOURNAL ARTICLE published 1 January 2018 in Biology Methods and Protocols

Authors: Kosuke Ishikawa | Yuta Kobayashi | Yutaro Wakabayashi | Shinya Watanabe | Kentaro Semba

Getting it right: Teaching undergraduate biology to undermine racial essentialism

JOURNAL ARTICLE published 10 January 2023 in Biology Methods and Protocols

Authors: Elaine Guevara | Shyamalika Gopalan | Dashiell J Massey | Mayowa Adegboyega | Wen Zhou | Alma Solis | Alisha D Anaya | Steven E Churchill | Joseph Feldblum | Richard R Lawler

CurSa: scripts to curate metadata and sample genomes from GISAID for analysis and display in nextstrain and microreact

JOURNAL ARTICLE published 10 January 2023 in Biology Methods and Protocols

Authors: Luis Delaye

Sequence-specific inhibition of reverse transcription by recombinant CRISPR/dCas13a ribonucleoprotein complexes in vitro

JOURNAL ARTICLE published 22 January 2021 in Biology Methods and Protocols

Authors: Toshitsugu Fujita | Shoko Nagata | Miyuki Yuno | Hodaka Fujii

DNA base flipping analytical pipeline

JOURNAL ARTICLE published 1 January 2017 in Biology Methods and Protocols

Research funded by Deutsche Forschungsgemeinschaft (DFG CA 198/7,DFG CA 198/10)

Authors: Peng Zhang | Florian D. Hastert | Anne K. Ludwig | Kai Breitwieser | Maria Hofstätter | M. Cristina Cardoso